| To sterilized seawater, add 1 mL per 1 L of seawater of prepared solutions 1-6 after combining 3 and 4 together prior to addition | |||
|---|---|---|---|
| Solution Components | |||
| Solution 1: Nitrogen | Quantity (grams) | Quantity (X2) | Quantity (X3) |
| Deionized water | 1L | 2L | 3L |
| Ammonium chloride (NH4Cl) | 26.75 g | 53.49 g | 80.24 g |
| Solution 2: Phosphate | Quantity (grams) | Quantity (X2) | Quantity (X3) |
| Deionized water | 1L | 2L | 3L |
| Sodium Phosphate. Dibasic, 12-Hydrate, Crystal (Na2HPO4*12H2O) | 0.4 | 0.8 g | 1.2 g |
| Solution 3: Iron (Combine with 4 immediately prior to addition to seawater) | Quantity (grams) | Quantity (X2) | Quantity (X3) |
| Deionized water | 1L | 2L | 3L |
| Ferrous sulfate (FeSO4*7H2O) | 0.278 g | 0.556 g | 0.834 g |
| Solution 4: EDTA (Combine with 3 immediately prior to addition to seawater) | Quantity (grams) | Quantity (X2) | Quantity (X3) |
| Deionized water | 1L | 2L | 3L |
| Disodium Ethylenediamine Tetraacetate (Na2EDTA) | 3.72 g | 7.44 g | 11.16 g |
| Solution 5: Manganese | Quantity (grams) | Quantity (X2) | Quantity (X3) |
| Deionized water | 1L | 2L | 3L |
| Manganese Chloride (MnCl2) | 0.0198 g | 0.0396 g | 0.0594 g |
| Solution 6: Vitamins (Store in freezer) | Quantity (grams) | Quantity (X2) | Quantity (X3) |
| Deionized water | 1L | 2L | 3L |
| Thiamine | 0.2 g | 0.4 g | 0.6 g |
| Biotin | 0.001 g | 0.002 g | 0.003 g |
| Vitamin B12 | 0.002 g | 0.004 g | 0.006 g |
